Vayntrub made her acting debut appearing in three episodes of the NBC television series ER in 1995. She and Stevie Nelson teamed up to start the YouTube comedy channel Live Prude Girls where they produced a number of shorts. Live Prude Girls went on to be featured on NewMediaRockstars‘ Top 100 Channels countdown, ranked at number 93. Vayntrub has had small roles in film and television, including Life Happens and Silicon Valley. She has also starred in several CollegeHumor videos.

From 2013 to 2016, Vayntrub portrayed a saleswoman named Lily Adams in a series of TV commercials for AT&T. She returned to the role in 2020. She also portrayed Tina Shukshin as a series regular on the Yahoo! Screen series Other Space, created by Paul Feig, in 2015. The following year, she appeared in Feig’s Ghostbusters reboot alongside Other Space cast members Neil Casey, Eugene Cordero, Bess Rous, and Karan Soni. Between 2016 and 2017, she appeared in the television series This Is Us, playing Sloane Sandburg. In July 2017, Vayntrub was cast in the lead role of Marvel Comics superheroine Squirrel Girl in the New Warriors television pilot for Freeform. Freeform ultimately passed on the series that November, and despite attempts to move the series to another network, it was ultimately cancelled. Despite the series’ cancellation, Vayntrub voiced Squirrel Girl in the Marvel Rising franchise. She reprised the role for a six-episode scripted podcast from Marvel and Sirius XM titled Marvel’s Squirrel Girl: The Unbeatable Radio Show. Also in 2017, Vayntrub starred in the Eko interactive streaming television series That Moment When as Jill.

She confirms what we suspected all along . Check out the video below